Services and Materials for Agriculture Research and Technology "SMART Agrisolutions"
For increasing productivity, up-scaling efficiency and strengthening sustainability in agriculture
For increasing productivity, up-scaling efficiency and strengthening sustainability in agriculture
| Instrument details: | |
|---|---|
| Type: | Fast protein liquid chromatography |
| Make: | Akta purifier 10 |
| Model No: | GE Healthcare Bio sciences |
| Essential specification: | |
| Measurement Range: | 190-700 nm in step of 1 nm |
| Temperature Range: | +4 to +60 °C |
| Flow Rate: | 0–100 ml/min |
| Absorbance range: | 0.001–5.0 AU (full scale) |
| Software Name: | UNICORN |
| Sample type: | Liquid |
| Minimum sample volume: | 1ml |
| Accuracy: | ± 2 nm |
| Precision / Repeatability: | ± 0.01 nm |
| Result Type: | Graph/ Chromatogram |
| Flow rate range; Isocratic and gradient mode: | 0.05–20 ml/min in steps of 10 µl/min 0.1–20 ml/min in steps of 10 µl/min |
| Pressure range: | 0–5 MPa (50 bar, 725 psi) |
| Absorbance range: | 0.01–5.0 AU (full scale) |
| Wavelengths Hg lamp, fixed by changing filter Zn lamp: | 254 and 280 nm 313, 365, 405, 436 and 546 nm 214 nm |
| Max. pressure: | 4.0 MPa (40 bar, 580 psi) |
| Cell volume: | 2 µl (30 µl detector volume) |
| Conductivity range: | 1 µS/cm to 999.9 mS/cm |
| pH range: | 0 to 14 |
| Working principle: | |
| Fast protein liquid chromatography (FPLC) is a type of liquid chromatography that provides high resolution by small-diameter stationary phases for protein characterization and separation. In fast protein liquid chromatography, the solvent velocity is controlled by a microprocessor through a software interface to maintain the constant flow rate of the solvents. The mobile phase is an aqueous buffer, and the flow rate is kept constant by a positive-displacement pump while the buffer composition can be varied by drawing fluids from external reservoirs. The stationary phase is composed of beads, usually of cross-linked agarose, packed into a cylindrical column. The eluent is passed through the detectors to measure the salt concentration (by conductivity) and protein concentration (by absorbing the ultraviolet light at a wavelength of 280nm) | |
| E-manual: | |
| https://btiscience.org/wp-content/uploads/2014/04/FPLC_SystemManual.pdf | |
