TERI has developed the mycorrhizal - biofilm technology to enhance mycorrhization and sporulation. These biofilms can be initiated with low CFU of established bacteria. In addition to increase in mycorrhizal sporulation, these bacteria also possess multiple plant growth enhancing capability. Hence, some of them can directly be applied to plants and solutions can be tailored to suit specific requirements.

S.No.	Bacterial species	Compatible Mycorrhiza	Product info	Bacterial info.
1	Izhakiella australiensis	Rhizophagus irregularis	Contamination free in vitro produced product	Starter CFU - 4 X 106 CFU interacting with hyphae and ~300 spores. Contamination free and molecularly characterized Native to endo-mycorrhizae (naturally existing) Biofilm formation (units): 7.78±0.31 in biofilm mode; 11.12±0.45 in pure culture Phosphate solubilization (µg/mL): 5.67±0.57 in biofilm mode; 13.26±2.30 in pure culture Siderophore production
2	Phyllobacterium myrsinacearum	Rhizophagus irregularis	Contamination free in vitro produced product	Starter CFU - 4 X 106 CFU interacting with hyphae and ~300 spores. Contamination free and molecularly characterized Native to endo-mycorrhizae (naturally existing) Enhance AMF sporulation by 1.5X Biofilm formation (units): 6.95±0.61 in biofilm mode; 6.05±0.12 in pure culture Phosphate solubilization (µg/mL): 5.98±0.32 in biofilm mode; 0.24± 0.10 in pure culture Potassium solubilization Nitrogen fixation IAA and siderophore production Biocontrol against: Fusarium sp.
3	Terribacillus saccharophilus	Rhizophagus irregularis	Contamination free in vitro produced product	Starter CFU - 4 X 106 CFU interacting with hyphae and ~300 spores. Contamination free and molecularly characterized Native to endo-mycorrhizae (naturally existing) Enhances AMF sporulation Biofilm formation (units): 9.98±0.60 in biofilm mode; 7.27±0.42 in pure culture (GS) Phosphate solubilization (µg/mL): 10.21±1.78 in biofilm mode (Bf); 18.95±1.92 in pure culture Potassium and phytate solubilization IAA and siderophore production Biocontrol against: Fusarium sp., Rhizoctonia sp. and Colletotrichum sp.
4	Bacillus filamentosus	Rhizophagus irregularis	Contamination free in vitro produced product	Starter CFU - 4 X 106 CFU interacting with hyphae and ~300 spores. Contamination free and molecularly characterized Native to endo-mycorrhizae (naturally existing) Enhance AMF sporulation by 1.5X Biofilm formation (units): 7.40±0.82 in biofilm mode; 8.64±0.39 in pure culture Phosphate solubilization (µg/mL): 5.82±1.13 in biofilm mode); 12.41±4.07 in pure culture Phytate and Potassium solubilization IAA and siderophore production Biocontrol against: Rhizoctonia sp.
5	Bacillus filamentosus	Rhizophagus irregularis	Contamination free in vitro produced product	Starter CFU - 4 X 106 CFU interacting with hyphae and ~300 spores. Contamination free and molecularly characterized Native to endo-mycorrhizae (naturally existing) Biofilm formation (units): 7.12±0.80 in biofilm mode; 15.27±1.00 in pure culture Phosphate solubilization (µg/mL): 5.60±0.75 in biofilm mode; 2.55±0.74 in pure culture Nitrogen fixation Siderophore production

Key Features of Product:

• Uniform distribution of bacteria onto mycorrhizal structures with formation of bacterial biofilm
• Biofilms remain active and stable upto 8 months
• Tolerance to harsh environmental conditions and fluctuations
• Stable and performs well under nutrient stress such as Carbon and Nitrogen
• Can mobilize nutrients such as Phosphate and Potassium
• Contamination-free
• Compatible with seed coating polymers
• Compatible with diverse - mycorrhiza to form cross-kingdom biofilms
• Compatible with seeds directly to form mono-species biofilms
• Compatible with fungicides and chemical biostimulants

Target Crops?

• All field crops ⚊ Cereals, Pulses, Millets, Vegetables, Fodder or other grasses

Product can be applied by:

• Seed Coating
• Drip, micro sprinklers or overhead irrigation systems
• Streaming nozzle and apply easily down the rows
• Boom spray onto wet soil or pasture