Microencapsulation Technology for Non-Sporulating Species

Next Generation Micro-Encapsulation Technology for improving shelf life of commercially relevant non-sporulating bacteria

The Microencapsulation technology provide solution for the issue of reduced viability of non-sporulating bacteria (that is known to have a lower shelf life) during storage, transport and application. Non-sporulating bacteria are those that are unable to form spores (exo-spores or endo-spores). In comparison to sporulating bacteria (Bacillus sp.), non-sporulating bacteria (Bradyrhizobium, Rhizobia, Azospirillum) are not resistant to stress conditions such as temperature and humidity. In high temperatures, non-sporulating organisms become low efficient, which is one of the major constraints in sustaining shelf life of these organisms for longer durations as a microbial inoculant based productivity.

This next generation microencapsulation technology of TERI for nodulating rhizobacteria (non-sporulating bacteria) species using natural polysaccharides will lead to desired size of microspheres loaded with microbes for seed coating applications with higher shelf life. This will also increase the survivability and functionality of the microbial strains and in its storage condition in different temperature (10-40°C) as well.

Technology offers dry bacterial product with a shelf life up to two (2) Years.

The microencapsulated non-sporulating bacteria from TERI technology have shelf life of 18-24 months and can be kept at different storage conditions.

Features of Micro-encapsulated formulations
  • Uniform particle sizes
  • Higher shelf life at 10- 40°C
  • No cold storage requirement
  • Free of contamination
  • Compatible with seed coating polymers, known carriers and existing technology including drip irrigation or in furrow application
Bacteria that can be encapsulated to increase shelf life and high temperature tolerance:
1. Rhizobium sp.
2. Bradyrhizobium sp.
3. Azoarcus sp.
4. Gluconacetobacter diazotrophicus
5. Azospirillumbrasilense
6. Azospirillumlipoferum
7. Azotobacterchroococcum
8. Pseudomonas aureofaciens
9. Pseudomonas chlororaphis
10. Pseudomonas fluorescens

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