Instrument details: |
Type: |
Flash Chromatography |
Make: |
YAMAZEN CORPORATION |
Model No: |
AI-580S & AKROS |
Essential specification: |
Measurement Range: |
Wavelength range-200-800nm |
Catridges: |
30 micron Silica particles |
Temperature ranges: |
Not Applicable |
Flow Rate: |
1ml to 80 ml |
Solvent: |
Normal grade Solvent(Hexane, Chloroform etc) |
Component: |
Required TLC data |
Software name: |
Chromatograh XY5-110511 |
Sample type: |
Liquid/Solid |
Minimum sample volume: |
1mg |
Accuracy: |
Better than +/-2% on NIST traceable latex standards. |
Precision / Repeatability: |
Better than +/-2% on NIST traceable latex standards. |
Result Type: |
Chromatogram |
Working principle: |
The principle is that the eluent which is a liquid, under gas pressure (normally nitrogen or compressed air) rapidly pushed through a short glass column. The glass column is packed with an adsorbent of defined particle size with large inner diameter. The most used stationary phase is silica gel 40 – 63 μm, but obviously packing with other particle sizes can be used as well. Particles smaller than 25 μm should only be used with very low viscosity mobile phases, because otherwise the flow rate would be very low. Normally gel beds are about 15 cm high with working pressures of 1.5 – 2.0 bars. Originally only unmodified silica was used as the stationary phase, so that only normal phase chromatography was possible.
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E-manual: |
http://yamazenusa.com/products/yflc-ai-series/yflc-ai-580s/ |