Services and Materials for Agriculture Research and Technology "SMART Agrisolutions"

For increasing productivity, up-scaling efficiency and strengthening sustainability in agriculture

CSLM – laser optical microscope

Instrument details:
Type: CSLM – laser optical microscope
Make: Zeiss, Germany
Model No: LSM 710
Essential specification:
Wavelength range 488 – 640 nm
Z-stacking Yes
Live imaging Yes
Channels 34
Resolution: 4x1 to 6144x6144 pixels
Scan zoom 0.6x to 40x
Software: Zen version 2010
Working principle:
Confocal microscopy is an optical imaging technique used to increase micrograph contrast and/or to reconstruct three-dimensional images by using a spatial pinhole to eliminate out-of-focus light or flare in specimens that are thicker than the focal plane. Confocal microscope has the capability of isolating and collecting a plane of focus within a sample, thus eliminating the out of focus "haze" normally seen with a fluorescent sample. Fine detail is often obscured by the haze and cannot be detected in a non-confocal fluorescent microscope. Confocal microscope has a stepper motor attached to the fine focus, enabling the collection of a series of images through a three-dimensional object. These images can then be used for a two or three dimensional reconstruction. Double and triple labels can be collected with a confocal microscope. Since these images are collected from an optical plane within the sample, precise localizations can be performed.
E-manual:
https://applications.zeiss.com/C125792900358A3F/0/E832638AA049A13BC12580570039275C/$FILE/EN_41_011_128_Axio-Observer_rel1-1.pdf
Applications:
Nanomaterial uptake: tagged nanomaterials can be detected for uptake in human cells, plant tissues, microbes and model organisms like nematodes and zebrafish,
Samples:
Sample preparation at center/ self-prepared sample
Type of samples live or fixed
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