Malvern Zetasizer Nano-ZS90

Instrument details:
Type: Dynamic light scattering
Make: Malvern, UK
Model No: Zetasizer Nano-ZS 90
Essential specification:
Measurement range: Size range = 0.3 nm – 5 µm (diameter)
Scattering angle: 90 ⁰C
Temperature ranges: 0-120 ⁰C
pH titrator: MPT-2 autotitrator
Laser fitted: He-Ne laser at 633nm
Software: Zetasizer Nano ZS
Narrow band filter, Universal ‘Dip’ cell & MPT-2 Auto-titrator present
Minimum sample volume: 20µL
Accuracy: Better than +/-2% on NIST traceable latex standards.
Precision / Repeatability: Better than +/-2% on NIST traceable latex standards.
Working principle:
The Zetasizer system determines the hydrodynamic radius of an equivalent spherical mass by first measuring the Brownian motion of the particles using Dynamic Light Scattering (DLS) and then determines the size of particles in a sample. The light source e.g., laser illuminates the sample in cuvette and the particles of the sample scatters the laser in all directions. This leads to the formation of bright and dark patches on the screen known as speckle pattern. Brighter areas are due to constructive interference of scattered light waves whereas darker patches of the speckle pattern are due to destructive interference of the scattered waves. The co-relation of this scattering intensity fluctuations w.r.t. rate of decay (time) determines the quality of particles in terms of big or small and then size is determined using Stokes- Einstein equation.
Nanomaterial characterization: Hydrodynamic size, surface potential, colloidal stability, aggregation index, poly dispersity index.
Sample preparation at center/ self-prepared sample
Type of samples polymeric/metallic/peptide or protein
Type of solvent organic/ inorganic solvent
Analysis Hydrodynamic diameter, zeta potential, pH titration
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